Tthssb
WebAug 8, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand WebAfter binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily. SSB is involved in DNA replication and …
Tthssb
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http://www.diva-portal.org/smash/record.jsf?pid=diva2:658627 Web(A and B) Representative single-molecule time traces (A) and average cross-correlation curves (B), for (dT)65+hp+3 alone and TthSSB-bound (dT)65+hp+3, obtained at 500 mM …
WebJun 21, 2006 · It is shown that DgeSSB is similar to Thermus/Deinococcus SSB in its biochemical properties and offers an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and for analytical purposes. To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus … WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse spontaneously along ssDNA over a wide range of salt concentrations (20-500mM NaCl), and that TthSSB diffusion can help transiently melt the DNA hairpin structures.
WebAug 10, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the … WebTthSSB appears to be positioned near the center of the (dT)60+n ssDNA overhangs though rapid diffusion. (A) A schematic illustration of our experimental design for (dT)60+n (n = 0, …
WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95°C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes.
WebOct 1, 2002 · TthSSB and TaqSSB monomer proteins contain 264 and 266 amino acids, respectively.The homology between these proteins is very high—82% identity and 90% … five below pop it fidgetWebOct 1, 2002 · The nucleotide (nt) sequence revealed that T. thermophilus SSB (TthSSB) and T. aquaticus (TaqSSB) consist of 264 and 266 amino acids, respectively, and have a molecular weight of 29.87 and 30.03kDa, respectively. The homology between these protein, is very high-82% identity and 90% similarity. They are the largest known prokaryotic SSB … canine massage guild therapistsWebJun 21, 2006 · In a complementation assay in Escherichia coli, DgeSSB took over the in vivo function of EcoSSB. DgeSSB is thermostable with half-lives of 50 min at 70 degrees C and 5 min at 90 degrees C. Hence, DgeSSB offers an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and for analytical purposes. canine marijuana toxicity symptomsWebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical … canine massage schoolWebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not … canine massage guild therapist registerWebNov 15, 2003 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus. TthSSB was also able to bind single-stranded RNA, … canine massage guild ukhttp://www.biotools.eu/documentospdf/DNA-Polymerase-030.pdf canine marking behavior