Phis2-p53

Webb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。 材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基 Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His …

pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …

Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by … Webb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … flamingo how to say https://omnigeekshop.com

新-TF-centered Y1H筛选与转录因子A相互作用的motif实验结题报 …

http://www.engineeredprocess.com/images/Honeywell_IFM_P531.P532_IOM.pdf Webb15 nov. 2024 · To eliminate the false positive results of the TF-centered Y1H assay, the bait plasmid pGADT7-bZIP44 was verified for self-activation. pGADT7-bZIP44 + pHIS2-p53 and pGAD53m + pHIS2-p53 were respectively transformed into the Y187 yeast cells, which were spread on SD/-Trp-Leu medium and incubated at 30 ℃ for 3–5 d. http://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 can printer be plugged into ups

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Phis2-p53

LkARF7 and LkARF19 overexpression promote adventitious root …

Webbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees … Webb人们最初认为p53基因是一种癌基因，但随着近十年研究的深入， p53 作为抑癌基因的功能逐渐被揭示出来。 在人类50%以上的肿瘤组织中均发现了p53基因的突变，这是肿瘤中 …

Phis2-p53

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Webb实验步骤: 1.构建载体目的基因融合连入pGADT7-Rec2 (AD); 顺式作用元件连入pHis2或pHis2.1载体 (顺式元件可以只连接一个拷贝， 也可以连接3个串联的并测序确认无误)。. 2.验证两载体分别于对应的空载体共转时，有无激活转录报告基因情况，大多酵母苗株有毒 … http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html

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Webb24 jan. 2024 · The p53 fragment was from pGBKT7-p53 used for Y2H. ... The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. WebbThe corresponding transformation with pGADT7 prey vector containing WRKY18, WRKY40, or WRKY60 and pHIS2 bait vector containing p53 promoter fragment were used as negative controls.

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: …

Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 … flamingo incident in the bahamasWebb4 okt. 2013 · The interactions of pHIS2-p53 (three tandem copies of the cis-acting DNA consensus sequence in pHIS2, which is recognized by p53) with the tested TFs were used as negative controls. Two MYC ( ThMYC4 and ThMYC6 GenBank number: JN166788 and JN166790) were identified to bind to the E-box motif, of which ThMYC6 that bound with … can printed paper be compostedWebbpHIS2-p53+pGADT7-53m（阳性对照） pHIS2+pGADT7-53m（阴性对照） 服务特色：经验足 周期短 性价比高 通量高. 客户提供. 构建酵母文库材料. 细胞样本：细胞数量＞ … can printer ink cartridges freezeWebbPGADT7-p53 binding to pHIS2-p53 (AD-Rec2-p53+ pHIS2-p53) used as the positive control, AD-Rec2-ScAPD1-like and pHIS2-p53 were used as the negative control. Source publication can printer be connected to laptopWebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without … can printer ink cartridges be refilledhttp://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html flamingo innovations gmbhWebb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 … flamingo in adopt me rarity