Fitc anti-mouse/human cd45r/b220
WebFeb 10, 2024 · FITC anti-mouse/human CD45R/B220 Antibody. Catalog Number: 103205. Clone Number: RA3-6B2. Info:Supplier Page View Product Specs View Product. Browse Similar Products: B220 Flow … Web3. Add Mouse BD Fc Block™ at 0.25 µg/10e6 cells, and incubate on ice for 15 minutes. 4. Wash cells with at least an equal volume of 1X BD IMag™ buffer, and carefully aspirate all the supernatant. 5. Vortex the BD IMag™ anti-mouse CD45R/B220 Particles - DM thoroughly, and add 50 µl of particles for every 10e7 total cells. 6.
Fitc anti-mouse/human cd45r/b220
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WebCD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in … WebStaining of C57Bl/6 splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Human/Mouse CD45R …
WebView Mouse Monoclonal anti-B220/CD45R Antibody (HIS24) [FITC] (NBP1-44007). Validated Applications: Flow, IHC, IHC-Fr. Validated Species: Rat. ... Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of Anti-Rat CD45R (B220) FITC (filled...read more. Product Details. Summary. … WebDescription: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T ...
WebClone REA755 recognizes the mouse CD45R (B220) antigen, a B lineage-specific surface molecule, which is expressed on B lymphocytes throughout their development from early pro–B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells … WebAnti-mouse/human CD45 also known as B220. Functional overview of this B cell lineage and mature B cell marker; Anti-mouse CD45R antibody clone RA3-6B2 described.
WebCitations: (136) BioLegend's FITC anti-mouse/human CD45R/B220 is a Rat monoclonal antibody. The FITC anti-mouse/human CD45R/B220 Antibody was generated using PTPRC as the antigen. It reacts with Human, and Mouse. This antibody has been shown to work in applications such as: Flow Cytometry. FITC anti-mouse/human CD45R/B220 …
WebCD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: … how is diphtheria contractedWebThe RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most … how is direction of magnetic field determinedWebFeb 9, 2024 · CD45R Antibody (RA3-6B2) is an IgG 2a rat monoclonal CD45R antibody (also designated CD45 antibody, PTPRC antibody or protein tyrosine phosphatase receptor type C antibody) suitable for the detection of the CD45R protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. CD45R Antibody (RA3-6B2) is available as both … highlander steakhouse menuWebMulticolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody (Cat. No. 553062/553061/561827) and with either PerCP-Cy5.5 Rat IgG2a, κ Isotype Control (Cat. No. 550765; Left Plot) or PerCP-Cy5.5 Rat Anti-Mouse CD45R/B220 … highlander strange haze ipaWebMost mouse and human B cell panels should include cell surface markers CD19 or CD20. B220 can be used as a marker for mouse B cells and not a pan B cell marker in humans. ... Cells were then surface stained with anti-CD3 and with anti-B220 and intracellularly stained with Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody ... CD45R: Positive ... highlander streaming vostfrWebB220 (CD45R) Antibodies. Antibodies that detect B220 (CD45R) can be used in several scientific applications, including Flow Cytometry, Immunohistochemistry, … how is diphtheria transmittedWebFITC Mouse Anti-Human CD45. Flow cytometric analysis of CD45 expression on human peripheral blood lymphocytes. Human whole blood was stained with either FITC Mouse … how is direct current produced in batteries